September 10, 2025PeerJOpen Access

Analytical performance evaluation of a multiplex real-time RT-PCR kit for simultaneous detection of SARS-CoV-2, influenza A/B, and RSV

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Cross-sectional analysis demonstrates strong diagnostic accuracy of a multiplex RT-PCR kit for SARS-CoV-2, influenza A/B, and RSV detection.

The LabTurbo multiplex real-time RT-PCR kit achieved 100% positive and negative agreements with reference assays for viral detection.
In testing 350 nasopharyngeal samples, LODs were determined as 8,333 copies/mL for SARS-CoV-2 and 3,333 for influenza A.
Cross-sectional data confirms the kit's effectiveness in differentiating co-circulating viruses like SARS-CoV-2, influenza A/B, and RSV.
Rapid identification of these pathogens using the LabTurbo kit may enable timely treatment measures to control virus spread.

Tai et al. (2025) studied this question.

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Cross-sectional analysis demonstrates strong diagnostic accuracy of a multiplex RT-PCR kit for SARS-CoV-2, influenza A/B, and RSV detection.