Transcriptional Regulation of the Interferon Alpha‐Inducible Protein‐27 Like 2b by the Peroxisome Proliferator‐Activated Receptor γ in Fatty Liver Disease

Peroxisome proliferator‐activated receptor γ (PPARγ) is a nuclear receptor abundantly expressed in the fatty liver of type 2 diabetic ob/ob mice. Herein, we investigated how PPARγ regulates the expression of the interferon alpha‐inducible protein 27‐like 2b ( lfi27l2b ) gene in the mouse liver. High expression of lfi27l2b was observed in the fatty liver of ob/ob mice, and the expression was further upregulated by PPARγ ligands; however, liver‐specific Pparg knockout ameliorated this increase. Moreover, high lfi27l2b expression was observed in the fatty liver of alcohol‐fed mice. Reporter assays indicated that the PPARγ‐responsive element (PPRE) is necessary for PPARγ‐dependent induction of Ifi27l2b promoter activities. Furthermore, electrophoretic mobility shift assays showed that PPARγ is capable of directly binding the PPRE. Overall, our results indicate that lfi27l2b is a novel PPARγ target in the fatty liver.