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September 10, 2025Jentashapir Journal of Cellular and Molecular BiologyOpen Access

Impact of Lipemia and Hemoglobin S Variant on HbA1c Measurement: A Comparison of Diazyme Enzymatic Method and Capillary Electrophoresis

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Authors

ZLZahra LotfiSFSomayeh FarahmandSZSedigheh Zargani

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Overview

Analytical study compares HbA1c accuracy in diabetes using enzymatic method and capillary electrophoresis under lipemia, suggesting increased reliability of CE.

Key Points

  • Capillary electrophoresis proves to be more reliable than the enzymatic method for measuring HbA1c under severe lipemia.
  • Significant false elevation in HbA1c was observed with the enzymatic method at high lipemia (P < 0.01).
  • Saline washing did not mitigate lipemic interference in the enzymatic method, leading to further inaccuracies.
  • Capillary electrophoresis maintained stable results, indicating its higher resistance to matrix interferences.

Cite This Study

Lotfi et al. (2025) studied this question.

synapsesocial.com/papers/68c23caeb210217d6478a8f9https://doi.org/10.5812/jjcmb-162424
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