Standardizing single-cell approaches to osteoarthritis: Toward a comprehensive cellular atlas

Osteoarthritis (OA) is a degenerative joint disease characterized by progressive cartilage degradation and complex cellular heterogeneity. Over the past several years, single-cell RNA sequencing (scRNA-Seq) has emerged as a powerful tool to dissect the cellular composition of the human osteoarthritic joint. However, building a complete picture of the OA single-cell milieu in the articular cartilage from these early datasets is like assembling a puzzle from dozens of different sets, where the pieces differ in size and shape.