September 10, 2025GigaScienceOpen Access

Using synthetic RNA to benchmark poly(A) length inference from direct RNA sequencing

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Dorado is identified as the preferred tool for poly(A) length estimation due to low mean error and fast runtimes.
The four poly(A) estimation tools demonstrated performance variations influenced by length and sample dependencies.
Synthetic RNA standards with known poly(A) lengths were utilized to accurately benchmark existing tools against BoostNano.
Accurate poly(A) length inference is crucial for understanding transcript stability and regulatory mechanisms.

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Analysis of poly(A) length estimation tools, including BoostNano, tailfindr, and Dorado, highlights performance variations.

Chang et al. (2025) studied this question.

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