September 10, 2025HorticulturaeOpen Access

Selection and Validation of Reference Candidate Genes for qRT-PCR Analysis in the Developing Fruit of Phyllanthus emblica L.

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Identified PeACT and PeUBQ6 as suitable housekeeping reference genes for qRT-PCR analysis.
Comparison of expression profiles for 13 candidate genes indicated stability and efficiency at different fruit development stages.
Multiple statistical methods, including BestKeeper and geNorm, were employed to validate the reference genes.
This study supports future investigations into gene expression related to fruit development and ripening.

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This analysis identifies suitable housekeeping reference genes for qRT-PCR in Phyllanthus emblica fruit, suggesting improved gene expression studies.

Pu et al. (2025) studied this question.

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This analysis identifies suitable housekeeping reference genes for qRT-PCR in Phyllanthus emblica fruit, suggesting improved gene expression studies.