This research uncovers links between m6A methylation, R-loops, and DNA damage response in pediatric high-grade gliomas, indicating potential therapies.
Key Points
Dysregulation of m6A readers and anti-readers correlates with genomic instability in pediatric high-grade gliomas.
RT-qPCR revealed significant upregulation of FXR1 and CAPRIN1 in pediatric high-grade glioma cell lines.
Immunofluorescence indicated that m6A methylation and R-loops are associated with upregulation of DNA damage markers.
siRNA knockdowns of FXR1 and CAPRIN1 reduced invasion and proliferation in the studied cancer cells.