Faecal egg count reduction test, deep amplicon sequencing of isotype-1 β-tubulin gene and in ovo larval development assay reveal susceptibility to benzimidazoles of porcine nematodes Oesophagostomum spp. and Ascaris suum in outdoor-reared pigs in Germany
This analysis reveals anthelmintic resistance in Oesophagostomum is absent, while Ascaris suum shows ambiguous FECRT results, suggesting careful interpretation is needed.
Key Points
The faecal egg count reduction test revealed no anthelmintic resistance in Oesophagostomum on German farms, indicating effective treatment.
Amplicon sequencing showed no β-tubulin gene polymorphisms linked to resistance in Oesophagostomum, reinforcing its susceptibility profile.
The in ovo larval development assay identified all tested populations of Ascaris suum as susceptible to benzimidazoles, indicating treatment efficacy.
However, interpretation of FECRT for Ascaris suum was complicated due to potential false positives from coprophagy among pigs.