RAA-CRISPR/Cas12a-based visual field detection system for rapid and sensitive diagnosis of major viral pathogens in calf diarrhea

View Full Paper

Ask AI

Bookmark

Authors

Discussion

Viral detection system improves diagnosis of calf diarrhea in cattle, indicating higher sensitivity and portability.

The RAA-CRISPR/Cas12a system demonstrated 1.6–4.9 times higher detection rates compared to traditional PCR.
A clinical validation of 252 samples identified 239 positives using the new method, proving its efficacy.
This rapid diagnostic test can be conducted in just 40 minutes, significantly reducing time to detection.
The system's high sensitivity (1–10 copies/μL) and 100% specificity positions it as a valuable asset in veterinary diagnostics.

Chen et al. (2025) studied this question.

View Full Paper

Ask AI

Bookmark