This protocol outlines using barley stripe mosaic virus vectors to induce gene silencing in Nicotiana benthamiana, suggesting efficient gene study methods.
This protocol details the infiltration of modified Barley Stripe Mosaic Virus vectors from Yuan, C., et al. (2011). "A High Throughput Barley Stripe Mosaic Virus Vector for Virus Induced Gene Silencing (VIGS) in Monocots and Dicots." PLOS ONE 6(10): e26468. into Nicotiana benthamiana plants and then rub inoculation of the virus-infected sap into target plants to induce Virus Induced Gene Silencing / VIGS or Virus Mediated Overexpression / VOX. Further details see Lee, W.-S., et al. (2015). "Virus induced gene silencing (VIGS) for functional analysis of wheat genes involved in Zymoseptoria tritici susceptibility and resistance." Fungal Genetics and Biology 79: 84-88. http://dx.doi.org/10.1016/j.fgb.2015.04.006 Mellado-Sánchez, M., et al. (2020). "Virus-mediated transient expression techniques enable gene function studies in black-grass." Plant Physiology 183(2): 455-459. Accession Number: 32238443 PMCID: PMC7271810 DOI: DOI:10.1104/pp.20.00205 Mellado-Sánchez, M., et al. (2020). "Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroides." bioRxiv: 2020.2001.2028.923466 DOI: 10.1101/2020.01.28.923466 Bouton, C., et al. (2018). "Foxtail mosaic virus: A Viral Vector for Protein Expression in Cereals." Plant Physiology 177(4): 1352-1367. http://dx.doi.org/10.1104/pp.17.01679
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Mellado-Sánchez et al. (2024) studied this question.