Long Non-Coding RNA 74687 Regulates Meiotic Progression and Gonadal Development in Rainbow Trout (Oncorhynchus mykiss) via the miR-15a-5p–ccne1 Regulatory Axis
Functional analysis reveals lncRNA74687 enhances CCNE1 expression in rainbow trout, suggesting its importance in gonadal development and meiotic progression.
Key Points
Knockdown of lncRNA74687 led to a 36.4% reduction in proliferation of gonadal cells, revealing its crucial role in cell growth and development.
Using dual-luciferase assays, lncRNA74687 was found to target CCNE1 and was regulated by miR-15a-5p, highlighting their interconnected functions.
Transcriptomic profiling indicated a broad downregulation of meiosis-related genes in lncRNA74687-deficient gonads, emphasizing its importance in germ-cell regulation.
The lncRNA74687–miR-15a-5p–CCNE1 regulatory axis underscores the complex interactions in gonadal development during the meiotic process.