This analysis shows that deep eutectic solvents improve antioxidant activity in Prunus spinosa, indicating a novel extraction method's effectiveness.
Key Points
Choline chloride/formic acid (2/1) extraction achieved the highest total phenolic content yields of 52.34 mg-GAE/g-FF.
The study utilized Fourier-transform infrared spectroscopy to confirm deep eutectic solvent formation and its efficacy.
Using Box-Behnken Design methodology, the ideal conditions for maximizing bioactive extraction were determined to be 50% water and 69.906 seconds of extraction time.
This method may enable superior bioactive recovery compared to traditional aqueous ethanol extraction methods.