August 19, 2025Open Access

Characterizing the oral microbiota of adolescents: results from the Generation R Study

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Participants exhibited a high similarity in oral microbiota, sharing a core microbiota of 23 genera including streptococcus and rothia.
The analysis indicated that sampling time negatively impacted diversity, with morning and afternoon samples showing a β of -0.54.
Using 16S rRNA gene sequencing, the study assessed core microbiota composition, alpha diversity, and beta diversity.
This characterization emphasizes the need for adjusting studies for technical covariates linked to oral microbiota assessments.

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Observational analysis revealed similarities in oral microbiota among adolescents, implying technical factors influence data.

Veenman et al. (2025) studied this question.

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