Production, purification and In vitro anticancer efficiency of L-asparaginase obtained from bacterial isolate of black gram rhizospheric soil sample of J&K
Analysis reveals high specificity l-asparaginase production from bacterial isolate in rhizospheric soil, suggesting enhanced anticancer potential against various cell lines.
Key Points
L-asparaginase from microbial sources shows significant anticancer activity against human cell lines.
Purified enzyme inhibited growth in breast and pancreatic cancer cells, indicating its therapeutic potential.
Anion exchange purification using Biologic-Duoflow FPLC system maintained high enzyme activity and specificity.
Investigating bacterial sources may reduce hypersensitivity reactions associated with traditional l-asparaginase therapies.