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August 18, 2025PLoS ONEOpen Access

CRISPR/Cas9 mediated ENT2 gene knockout altered purine catabolic pathway and induced apoptosis in colorectal cell lines

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Authors

SNSafaa M. NaesSASharaniza Ab‐RahimMMMusalmah Mazlan

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Overview

CRISPR/Cas9 knockout increases reactive oxygen species in colorectal cancer cells, suggesting new therapeutic avenues.

Key Points

  • Colorectal cancer cell lines showed increased apoptosis after ENT2 gene knockout, disrupting purine metabolism.
  • Significantly elevated levels of hypoxanthine and xanthine oxidase activity were measured in knockout clones.
  • CRISPR/Cas9 technology was used for gene editing, targeting the ENT2 gene in colorectal cancer cells.
  • Targeting ENT2 may provide new strategies for increasing apoptosis in colorectal cancer treatments.

Cite This Study

Naes et al. (2025) studied this question.

synapsesocial.com/papers/68af2d83cf1dd9ea359e5b7ahttps://doi.org/10.1371/journal.pone.0329501
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