July 24, 2025

Nested PCR methodology for early detection of Lasiodiplodia sp. and Ceratocystis sp. in avocado and cocoa seedling samples

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Nested PCR methodology successfully identifies Ceratocystis and Lasiodiplodia in avocado and cocoa seedlings.
Specific primer sets demonstrated sensitivity of 2–4 ng/µL for accurate pathogen detection.
Pre-treatment of samples with glycerol and ethanol improved DNA isolation from plant tissues.
The technique avoids false negatives and allows diagnosis without previous fungal culture, enhancing plant health management.

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Nested PCR detects Ceratocystis and Lasiodiplodia in asymptomatic cocoa and avocado seedlings, suggesting effective early diagnosis.

Laverde-Arias et al. (2025) studied this question.

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